Electrochemical biosensor and the manufacturing method thereof
AssigneesChina Medical University, China Medical University Hospital
InventorsYi-Wen Chen, Yi-An Chen, Ming-You Shie
Life of this patent
Estimating the term…
Abstract
An electrochemical biosensor has an electrode which is sequentially decorated a molybdenum (VI) oxide (MoO3) nanomaterial, a gold-composite nanomaterial, a carboxyl-sulfhydryl compound, and a carbodiimide/N-hydroxysuccinimide (EDC/NHS, EN) to modify the surface of the electrode. That the sulfhydryl group of the carboxyl-sulfhydryl compound forms a bond with the gold-composite nanomaterial, and the carbodiimide/N-hydroxybutanediimide forms a bond with a carboxyl group of the carboxyl-sulfhydryl compound.
Claims6
An electrochemical biosensor, consisting essentially of a working electrode with a surface provided with a molybdenum trioxide nanomaterial and a gold nanocomposite disposed over at least a portion of the molybdenum trioxide nanomaterial for modifying the working electrode, and a carboxyl-mercapto compound and a N-3-dimethylaminopropyl-N′-ethylcarbodiimide hydrochloride/N-hydroxysuccinimide (EDC/NHS, EN) for modification, wherein,
- bonds form between a mercapto group of the carboxyl-mercapto compound and the gold nanocomposite, and the N-3-dimethylaminopropyl-N′-ethylcarbodiimide hydrochloride/N-hydroxysuccinimide (EDC/NHS, EN) bonds with a carboxyl group of the carboxyl-mercapto compound,
- marker is attached to the N-3-dimethylaminopropyl-N′-ethylcarbodiimide hydrochloride/N-hydroxysuccinimide (EDC/NHS, EN), and
- the gold nanocomposite is formed by gold nanoparticles coated on a surface of a metal oxide nanomaterial, wherein
- the metal oxide nanomaterial comprises manganese dioxide (MnO2) or zinc oxide (ZnO).
Dependencies are inferred from the claim text.