Microscope for high-resolution and specific analysis of biological substances, and method of analysis

Abstract

The subject of the invention is a microscope for high-resolution and specific analysis of biological substances. The microscope according to the invention is characterized by a power supply unit capable of generating constant current attached to the microscope housing; a set of mirrors and illuminating optics; a collimator placed between a monochromatic light source emitting light in a very narrow range of wavelengths and the collector lens; a dark-field condenser and objective including an in-between space to accommodate the stained biological specimen; and a surveillance camera or eyepiece, and a removable color filter placed between the objective and the surveillance camera or the eyepiece. The invention also covers the method to analyze biological specimens with fluorescent staining, wherein the biological contents of the specimen are analyzed in a growing medium in liquid state after the addition of antibodies marked with fluorescent stain, using the microscope according to the invention. The microscope according to the invention enables up to one order of magnitude higher resolution than conventional light microscopes and 10-30% higher resolution compared to currently used dark-field microscopes and provides a remarkably sharper camera image.

Dependencies are inferred from the claim text.