Assembly comprising a nucleic acid scaffold, systems, methods, and uses therefore
AssigneeOxford University Innovation Limited
InventorsHagan Bayley, Evan Spruijt, Anja Henning-Knechtel
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Abstract
The invention provides an assembly comprising a nucleic acid scaffold and a plurality of appendages bonded to the scaffold, wherein the appendages, when bonded to the scaffold, are capable of interacting to form a pore in a layer of amphipathic molecules.
Claims30
An assembly comprising a nucleic acid scaffold and a plurality of appendages bonded to the scaffold, wherein the appendages form a pore in a layer of amphipathic molecules, and wherein the nucleic acid scaffold comprises a plurality of nucleic acids arranged to form a closed circular structure with protruding arms.
A system comprising:
- (a) a layer of amphipathic molecules;
- and (b) an assembly comprising a nucleic acid scaffold and a plurality of appendages bonded to the scaffold, wherein the appendages form a pore in the layer of amphipathic molecules, and wherein the nucleic acid scaffold comprises a plurality of nucleic acids arranged to form a closed circular structure with protruding arms.
A method of forming a pore in a layer of amphipathic molecules, the method comprising applying an assembly to a layer of amphipathic molecules, wherein the assembly comprises a nucleic acid scaffold and a plurality of appendages bonded to the scaffold, wherein the nucleic acid scaffold comprises a plurality of nucleic acids arranged to form a closed circular structure with protruding arms, and the appendages to interact to form a pore in the layer of amphipathic molecules.
A method of detecting and/or characterising a target analyte, comprising:
- (a) forming a pore in a layer of amphipathic molecules by applying an assembly to a layer of amphipathic molecules, wherein the assembly comprises a nucleic acid scaffold and a plurality of appendages bonded to the scaffold, wherein the nucleic acid scaffold comprises a plurality of nucleic acids arranged to form a closed circular structure with protruding arms, and the appendages to interact to form a pore in the layer of amphipathic molecules;
- (b) contacting the target analyte with the pore;
- and
- (c) taking one or more measurements as the target analyte moves through the pore, wherein the measurements are indicative of the presence of and/or one or more characteristics of the target analyte.
An assembly comprising a nucleic acid scaffold and a plurality of appendages bonded to the scaffold, wherein the appendages form a pore in a layer of amphipathic molecules, and wherein the nucleic acid scaffold comprises (i) a closed circular first nucleic acid strand and (ii) a plurality of second nucleic acid strands, wherein the second nucleic acid strands comprise a first region that hybridises to the first nucleic acid strand and a second region that protrudes from the circular first nucleic acid strand.
A system comprising:
- (a) a layer of amphipathic molecules;
- and (b) an assembly comprising a nucleic acid scaffold and a plurality of appendages bonded to the scaffold, wherein the appendages form a pore in the layer of amphipathic molecules, and wherein the nucleic acid scaffold comprises (i) a closed circular first nucleic acid strand and (ii) a plurality of second nucleic acid strands, wherein the second nucleic acid strands comprise a first region that hybridises to the first nucleic acid strand and a second region that protrudes from the circular first nucleic acid strand.
Dependencies are inferred from the claim text.