NAD(P)- dependent responsive enzymes, electrodes and sensors, and methods for making and using the same
AssigneeAbbott Diabetes Care Inc.
InventorsTianmei Ouyang, Benjamin J. Feldman
Life of this patent
Estimating the term…
Abstract
NADP-dependent oxidoreductase compositions, and electrodes, sensors and systems that include the same. Analyte sensors include an electrode having a sensing layer disposed thereon, the sensing layer comprising a polymer and an enzyme composition distributed therein. The enzyme composition includes nicotinamide adenine dinucleotide phosphate (NAD(P)+) or derivative thereof, an NAD(P)+-dependent dehydrogenase; an NAD(P)H oxidoreductase; and an electron transfer agent comprising a transition metal complex.
Claims23
An electrochemical analyte sensor configured for partial insertion below a subject's skin surface, the sensor comprising:
- a portion configured to be positioned above the subject's skin surface and an inserted portion configured to be positioned below the subject's skin surface, wherein the inserted portion comprises:
- a non-conductive material layer;
- a first conductive layer disposed on at least a portion of the non-conductive material layer, wherein the first conductive layer is a working electrode;
- an enzyme composition disposed on a surface of the first conductive layer, wherein the enzyme composition comprises:
- a) nicotinamide adenine dinucleotide (phosphate) (NAD(P)+) or derivative thereof;
- b) D-3-hydroxybutyrate dehydrogenase;
- c) diaphorase;
- d) an electron transfer agent;
- and
- e) a polymer, wherein the NAD(P)+ or derivative thereof is not covalently bonded to the polymer;
- and
- a mass transport limiting membrane disposed over the enzyme composition to limit flux of D-3-hydroxybutyrate to the working electrode.
Dependencies are inferred from the claim text.