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US 12,698,495 B2Granted
Engineered tryptophan decarboxylases and uses thereof for synthesizing tryptamine analogs
AssigneeWisconsin Alumni Research Foundation
InventorsAndrew Buller, Allwin McDonald
Life of this patent
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Abstract
Unnatural, mutant tryptophan decarboxylase proteins and methods of using same. The mutants have an amino acid sequence at least 80% identical to SEQ ID NO:1 and have substitutions at one or more of amino acid positions 98, 99, 120, 126, 339, 349, and 355 of SEQ ID NO:1. The mutants can have enhanced activity and/or substrate promiscuity and be used for synthesizing tryptamine analogs.
Claims21
An unnatural, mutant protein comprising an amino acid sequence at least 90% identical to SEQ ID NO:1, wherein:
- the amino acid sequence comprises one or more of:
- a residue other than phenylalanine at a position corresponding to position 98 of SEQ ID NO:1;
- a residue other than valine at a position corresponding to position 99 of SEQ ID NO:
- 1;
- a residue other than histidine at a position corresponding to position 120 of SEQ ID NO:1;
- a residue other than leucine at a position corresponding to position 126 of SEQ ID NO:1;
- a residue other than leucine at a position corresponding to position 339 of SEQ ID NO:1;
- a residue other than tryptophan at a position corresponding to position 349 of SEQ ID NO:1;
- and
- a residue other than leucine at a position corresponding to position 355 of SEQ ID NO:
- 1;
- and
- the protein exhibits activity in decarboxylating a tryptophan analog substrate.
10 more elements
Dependencies are inferred from the claim text.