Isolated CAS13 protein and use thereof
AssigneesGuangzhou Reforgene Medicine Co., Ltd., Zhejiang Synsorbio Technology Co., Ltd.
InventorsJunbin Liang, Xingxiang Liang, Yang Sun, Hui Xu, Zhiqin Peng, Kaiwei Si, Desheng Huangfu
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Abstract
The present disclosure relates to an isolated Cas13 protein and use thereof. The amino acid sequence of the isolated Cas13 protein comprises a sequence having ≥50% sequence identity with the sequence as shown in any one of SEQ ID NO: 1-SEQ ID NO: 7, and SEQ ID NO: 60. The Cas13 protein is a Cas13 enzyme with an endonuclease activity, which can be used in a CRISPR/Cas system to achieve targeting and modification of a target nucleic acid, enriching the enzymes and systems available in a CRISPR-C as editing system.
Claims2
A non-naturally occurring conjugate comprising a Cas13 protein and a nuclear localization signal and/or a nuclear export signal;
- wherein the Cas13 protein comprises the amino acid sequence of SEQ ID NO:
- 3;
- wherein the Cas13 protein has Cas13 nuclease activity and can form a complex with a gRNA to bind to a target nucleic acid in a non-bacterial cell.
Dependencies are inferred from the claim text.