Loading patent…
AssigneeZhejiang University of Science and Technology
InventorsJun Huang, Tingting Cai, Shui Qiu, Lehe Mei, Jiaren Cao, Changjiang Lyu, Fangfang Fan, Sheng Hu, Ye Li, Weirui Zhao, Feng Wang, Yuanyuan Li
Estimating the term…
The amino acid sequence of a w-transaminase mutant derived from mutation of ω-transaminase from Aspergillus terreus, is as shown in SEQ ID NO. 4 or SEQ ID NO. 6. Compared with the wild-type enzyme, the half-lives of the ω-transaminase mutants are all above 24 h, while the half-life of the wild-type is only 6.90 min. The half-inactivation temperature of the mutants are 49.00° C. and 49.03° C., respectively, which are about 11° C. higher than that of the wild-type (37.89° C.), such that the thermal stability is significantly improved.
An ω-transaminase mutant based on ancestral sequence reconstruction, wherein the ω-transaminase mutant is derived from mutation of ω-transaminase from Aspergillus terreus, and the ω-transaminase mutant has the amino acid sequence of SEQ ID NO. 4 or SEQ ID NO. 6.
Dependencies are inferred from the claim text.