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US 12,698,481 B2Granted
Omega-hydroxylase-related fusion polypeptides with improved properties
AssigneeGenomatica, Inc.
InventorsBaolong Zhu, Andreas W. Schirmer, Cindy Chang
Life of this patent
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Abstract
The disclosure relates to omega-hydroxylase-related fusion polypeptides that result in improved omega-hydroxylated fatty acid derivative production when expressed in recombinant host cells. The disclosure further relates to microorganisms for ex pressing the omega-hydroxylase-related fusion polypeptides for the production of omega-hydroxylated fatty acid derivatives.
Claims21
A CYP153A-reductase hybrid fusion polypeptide variant, comprising at least 95% sequence identity to SEQ ID NO:
- 6 and comprising at least one mutation at an amino acid position corresponding to amino acid position 9, 10, 12, 13, 14, 28, 56, 61, 111, 119, 140, 162, 164, 204, 244, 254, 273, 302, 327, 413, 477, 480, 481, 527, 544, 546, 557, 567, 591, 648, 649, 703, 706, 707, 708, 709, 710, 719, 720, 736, 741, 745, 747, 749, 757, 770, 771, and 784 of SEQ ID NO:6, wherein said CYP153A-reductase hybrid fusion polypeptide variant catalyzes the conversion of a fatty acid or derivative thereof to an ω-hydroxy fatty acid or derivative thereof.
Dependencies are inferred from the claim text.