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US 12,698,470 B2Granted
Stability of microbial composition, and manufacturing methods therefore
AssigneeChr. Hansen A/S
InventorsPravin Gadkari, Furqan Ali, Surender Kumar Dhayal, Deepak Jain
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Abstract
The present invention relates to encapsulation of microbial cultures to improve the robustness and stability upon storage. In particular, the present invention relates to dry preparations of microbial cultures, such as lactic acid bacteria (LAB), coated by a fat-matrix that increase survivability and mitigate post-acidification upon storage at ambient temperature for extended periods of time.
Claims6
A method for the preparation of a composition comprising a microencapsulated microbial culture, said method comprising the steps of:
- a) obtaining a concentrated cell mass of the microbial culture;
- b) adding a protective compound to the concentrated cell mass of (a) to obtain a mixture;
- c) drying the mixture of (b) to obtain a dried mixture;
- d) optionally grinding the dried mixture of (c) to obtain a powder;
- e) coating the dried mixture of (c) or powder of (d);
- and
- f) mixing the coated dried mixture or powder of (e) with an excipient;
- wherein the protective compound is a cryoprotectant and/or lyoprotectant with a bulk density between 150 and 600 kg/m3.
5 more elements
Dependencies are inferred from the claim text.