Fungal cells for tailored fats

A fungal cell capable of producing triacylglycerols with fatty acids with an acyl chain having a target characteristic, wherein

• the fungal cell is genetically modified for expression of a heterologous lysophosphatidyl acyltransferase (LPAT) (EC 2.3.1.51) capable of esterifying a triacylglycerol precursor with an acyl-CoA with an acyl chain having the target characteristic;
• and
• the heterologous LPAT has preference for esterifying a triacylglycerol precursor with a saturated fatty acyl-CoA over esterifying a triacylglycerol precursor with an unsaturated fatty acyl-CoA,
• wherein the heterologous LPAT is selected from the group consisting of Bos taurus AGPAT4 as defined in SEQ ID NO:
• 13, 14, or 15, Bos taurus AGPAT1 as defined in SEQ ID NO:
• 16, Bos taurus AGPAT2 as defined in SEQ ID NO:
• 17, Bos taurus AGPAT3 as defined in SEQ ID NO:
• 18, Bos taurus AGPAT5 as defined in SEQ ID NO:
• 19, Arabidopsis thaliana LPAT1 as defined in SEQ ID NO:
• 20, Brassica napus LPAT1 as defined in SEQ ID NO:
• 24, Mycolicibacterium smegmatis ERS451418_00313 as defined in SEQ ID NO:
• 28, Mycolicibacterium smegmatis Probable LPAT as defined in SEQ ID NO:
• 29, Mycolicibacterium smegmatis ERS451418_06226 as defined in SEQ ID NO:
• 30, Mycolicibacterium smegmatis ERS451418_02370 as defined in SEQ ID NO:
• 31, Mycolicibacterium smegmatis ERS451418_05575 as defined in SEQ ID NO:
• 32, Mycolicibacterium smegmatis ERS451418_04128 as defined in SEQ ID NO:
• 33, Mycolicibacterium smegmatis ERS451418_06227 as defined in SEQ ID NO:
• 34, Mycolicibacterium smegmatis BIN_B_00519 as defined in SEQ ID NO:
• 35, Mycolicibacterium smegmatis D806_035910 as defined in SEQ ID NO:
• 36, Mycolicibacterium smegmatis BIN_B_03706 as defined in SEQ ID NO:
• 37 Mycolicibacterium smegmatis D806_003290 as defined in SEQ ID NO:
• 38, Cocos nucifera probable LPAT as defined in SEQ ID NO:
• 86, and a heterologous LPAT having at least 95% sequence identity to any of SEQ ID NOs:
• 13-20, 24, 28-38, or 86.