Woven cell culture substrates, bioreactor systems using the same, and related methods
AssigneeCorning Incorporated
InventorsAnn MeeJin Ferrie, Vasiliy Nikolaevich Goral, Lori Eileen Romeo
Life of this patent
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Abstract
A cell culture matrix for a perfusion-flow fixed-bed reactor is provided. The cell culture matrix includes a substrate having a porous sheet for adhering cells thereto. The sheet has a first side, a second side opposite the first side, a thickness separating the first side and the second side, and a plurality of openings formed in the substrate, arrayed in a regular pattern, and passing through the thickness of the substrate. The porous sheet is wound into a cylindrical shape having a plurality of wound layers, and the cell culture matrix does not include a spacer material or physical barrier between the plurality of wound layers of the substrate.
Claims18
A roller bottle system for cell culture, the roller bottle system comprising:
- a cell culture vessel comprising a culture chamber and a central longitudinal axis, the culture chamber being configured for containing cell culture media while the cell culture vessel is rotated about the central longitudinal axis;
- and
- a substrate disposed in the culture chamber, the substrate comprising a porous sheet configured for adhering cells thereto,
- wherein the porous sheet comprises a length and a width, the length extending in a direction parallel to the central longitudinal axis and the width being perpendicular to the length, and
- wherein the width of the porous sheet is greater than a circumference of an interior wall of the culture chamber.
A roller bottle system for cell culture, the roller bottle system comprising:
- a cell culture vessel comprising a culture chamber and a central longitudinal axis, the culture chamber being configured for containing cell culture media while the cell culture vessel is rotated about the central longitudinal axis;
- and
- a substrate disposed in the culture chamber, the substrate comprising a porous sheet configured for adhering cells thereto,
- wherein the substrate is unattached to the cell culture vessel.
A method of culturing cells in a roller bottle system, the method comprising:
- providing a roller bottle system comprising:
- a cell culture vessel comprising a culture chamber and a central longitudinal axis, the culture chamber being configured for containing cell culture media while the cell culture vessel is rotated about the central longitudinal axis;
- inserting a substrate through an opening in the roller bottle while the substrate is in a tightly wound state, wherein the substrate at least partially unwinds and expands once inside the culture chamber, such that the substrate is disposed in the culture chamber, the substrate comprising a porous sheet configured for adhering cells thereto;
- supplying cell culture media to the culture chamber through an opening in the cell culture vessel;
- seeding cells within the culture chamber such that the cells attach to the porous sheet;
- growing the cells within the culture chamber;
- and
- rotating the cell culture vessel about the central longitudinal axis during at least one of the seeding and the growing of the cells.
Dependencies are inferred from the claim text.