Tissue engineered scaffolds, instrumented bioreactors and methods of use thereof

A system, comprising:

• a housing comprising:
• a cavity housing component defining a cavity extending from an opening in a first surface of the cavity housing component;
• and
• a support housing component having a sealing surface configured to sealingly engage the first surface of the cavity housing component thereby sealing the opening and defining a fluid chamber having a sealing end defined by the support housing component,
• the support housing component defining a pair of intra-hollow tissue structure flow channels connected to the sealing end of the fluid chamber,
• the support housing component including a support ring configured to engage and support an open end of a hollow tissue structure at the sealing end of the fluid chamber with the hollow tissue structure suspended in the fluid chamber and supported only at the support ring, the hollow tissue structure having only one open end, and
• the support housing component configured to fluidly couple the pair of intra-hollow tissue structure flow channels with each other and with an interior of the hollow tissue structure via an intra-hollow tissue structure flow path at the sealing end of the fluid chamber;
• the housing defining a pair of extra-hollow tissue structure flow channels fluidly coupled to the fluid chamber, at least one of the pair of extra-hollow tissue structure flow channels defined by the cavity housing component, and
• the housing configured to receive the hollow tissue structure within the fluid chamber with the hollow tissue structure engaged and supported by the support ring at the sealing end of the fluid chamber to fluidly couple the pair of extra-hollow tissue structure flow channels via an extra-hollow tissue structure flow path extending between an exterior of the hollow tissue structure and a surface of the fluid chamber at least partially surrounding the hollow tissue structure.