Particle separation systems and methods
AssigneeLevitasbio, Inc.
InventorsTheodorus Evan de Groot, Geoff Facer, Amirreza Malekanfard, Gang Sun, Lesley Suzanne Weaver
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Abstract
Magnetic levitation particle separation systems and methods for use with multi-channel flow cells. The system may include a core for receiving and holding the flow cell, with upper and lower clamps for securing the flow cell and positioning it relative to an array of magnets. The system is configured to image particle separation in processing channels of the flow cell, and to regulate the flow cell's temperature. The core may be removable as a single unit, facilitating reconfiguration of the system.
Claims24
A multi-channel flow cell device, comprising:
- (a) a substrate;
- (b) a plurality of fluidic separation channels formed in the substrate, in which:
- (i) each fluidic channel comprises a magnetic processing channel in fluidic communication with an inlet and at least two outputs,
- (ii) each magnetic processing channel comprises at least one divider vertically separating a portion of the magnetic processing channel into at least an upper part and a lower part, the upper part in fluidic communication with one of the outputs and the lower part in fluidic communication with another of the at least two outputs,
- (iii) each magnetic processing channel further comprises at least one imaging surface extending along a length of the magnetic processing channel, the imaging surface configured to allow imaging of particles in the magnetic processing channel;
- and
- a plurality of openings extending through the substrate, wherein each opening extends between two adjacent magnetic processing channels, the openings configured for optical access to the at least one imaging surface of each magnetic processing channel;
- wherein the substrate and the plurality of fluidic separation channels formed in the substrate are configured as a multi-channel flow cell cartridge.
A multi-channel flow cell device, comprising:
- (a) a substrate;
- (b) a plurality of co-planar fluidic separation channels formed in the substrate, in which:
- (i) each fluidic channel comprises a magnetic levitation processing channel in fluidic communication with an inlet and at least two outputs,
- (ii) each processing channel comprises at least one divider vertically separating a portion of the processing channel into at least an upper part and a lower part, the upper part in fluidic communication with one of the outputs and the lower part in fluidic communication with the other of the outputs,
- (iii) each processing channel further comprises opposing side imaging surfaces extending along a length of the processing channel, the imaging surfaces configured to allow imaging of magnetic levitation of particles in the processing channel;
- the multi-channel flow cell device further comprising a plurality of openings extending through the substrate, wherein each opening extends between two adjacent processing channels, the openings configured for optical access to the imaging surfaces;
- wherein the substrate and the plurality of fluidic separation channels formed in the substrate are configured as a multi-channel flow cell cartridge.
Dependencies are inferred from the claim text.