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US 12,697,597 B2Granted
Methods for polynucleotide synthesis
AssigneeTwist Bioscience Corporation
InventorsJeremy Lackey, David Dodd, Stefan Pitsch
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Abstract
Provided herein are compositions, devices, systems and methods for DNA oligomer synthesis. Further provided are devices comprising addressable electrodes controlling polynucleotide synthesis (deprotection, extension, or cleavage, etc.). The compositions, devices, systems and methods described herein provide improved synthesis, storage, density, and retrieval of biomolecule-based information.
Claims20
A method of synthesizing a polynucleotide comprising:
- coupling at least one nucleoside attached to a solid support to a protected nucleoside, wherein the protected nucleoside comprises a protecting group, wherein the protecting group comprises a branched alkenylene, wherein the solid support comprises an addressable array, and wherein the addressable array comprises a plurality of loci;
- contacting an amount of a reagent with the protected nucleoside, wherein the contacting results in deprotection of the protected nucleoside;
- and
- repeating the coupling and contacting steps to synthesize the polynucleotide.
1 more element
Dependencies are inferred from the claim text.