Thrombus imaging aptamers and methods of using same
AssigneeDuke University
InventorsBruce A. Sullenger, Kady-Ann C. Steen-Burrell, Bethany Powell Gray
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Abstract
Provided herein are imaging agents, antidotes to the imaging agents and methods of using the same to image a thrombus or blood clot or thrombin including sites of thrombin accumulation and to diagnose and treat thrombosis. The imaging agents include an aptamer capable of binding the thrombus or thrombin in particular linked to a reporter moiety. The imaging agents may be used to label the thrombus or sites of thrombin accumulation. Antidotes capable of binding to the aptamer in the imaging agent are also provided. The antidotes may further be linked to a quencher capable of quenching the reporter moiety.
Claims17
An imaging agent comprising (a) an aptamer linked to (b) a reporter moiety, wherein said aptamer is capable of binding thrombin with a Kd<500 nM, wherein the aptamer comprises a nucleotide sequence having at least 70% sequence identity to SEQ ID NO:
- 1 or comprises a nucleotide sequence including from 5′ to 3′ a first stem forming region comprising at least three nucleotides, a first loop region comprising the nucleotide sequence AACA, a second stem forming region comprising at least three nucleotides, a second loop region comprising the nucleotide sequence C U/A GXAG U/A A, a third stem forming region comprising at least three nucleotides capable of forming a stem with the second stem forming region, a third loop region comprising at least one nucleotide and up to 5 nucleotides, and a fourth stem forming region comprising at least three nucleotides capable of forming a stem with the first stem forming region, wherein A is adenine, C is cytosine, G is guanine, U is uracil and X is any base in the nucleotide sequences.
Dependencies are inferred from the claim text.