Bacillus subtilis strain with probiotic activity
AssigneeEvonik Operations GmbH
InventorsStefan Pelzer, Daniel Petri, Christos Giatsis, Stella Molck, Maike Kipker, Jessica Kleinbölting, Lorena Stannek-Göbel, Kiran Doranalli, John Khun Kyaw Htoo, Claudia Borgmeier, Sandra Herbold, Guido Meurer
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Abstract
The current invention concerns a new B. subtilis strain with strong inhibition of swine and poultry related pathogens and its use as probiotic.
Claims15
A feed or foodstuff comprising:
- a) Bacillus subtilis DSM 32540;
- b) at least one ingredient selected from the group consisting of:
- proteins, carbohydrates, fats, additional probiotics, prebiotics, enzymes, vitamins, immune modulators, milk replacers, minerals, amino acids, coccidiostats, acid-based products, medicines, and combinations thereof;
- and
- c) at least one added carrier to improve one or more characteristics selected from the group consisting of:
- recovery;
- efficacy;
- physical properties;
- packaging and administration;
- wherein:
- i) when the feed or foodstuff is fed to an animal, the growth of pathogenic bacteria is reduced compared to growth in the feed or foodstuff without said Bacillus subtilis DSM 32540;
- and wherein said pathogenic bacteria are selected from the group consisting of:
- Clostridium perfringens, Clostridium difficile, Enterococcus cecorum, Streptococcus gallinaceus and Streptococcus suis bacteria;
- and
- ii) said Bacillus subtilis DSM 32540 is able to grow in the presence of 2 mM bile.
A feed or foodstuff comprising a naturally non-occurring mutant of Bacillus subtilis DSM 32540, wherein said mutant is not found in nature and comprises a DNA sequence identity to DSM 32540 of at least 99.5%;
- wherein:
- i) said mutant of Bacillus subtilis DSM 32540 inhibits the growth of at least one of:
- Clostridium perfringens, Clostridium difficile, Enterococcus cecorum, Streptococcus gallinaceus and Streptococcus suis bacteria;
- ii) said mutant of Bacillus subtilis DSM 32540 is able to grow in the presence of 2 mM bile;
- and
- iii) the feed or foodstuff further comprises at least one ingredient selected from the group consisting of:
- proteins, carbohydrates, fats, additional probiotics, prebiotics, enzymes, vitamins, immune modulators, milk replacers, minerals, amino acids, coccidiostats, acid-based products, medicines, and combinations thereof and an added carrier selected from the group consisting of:
- anti-caking agents;
- anti-oxidation agents;
- bulking agents;
- and protectants;
- wherein the mutant of Bacillus subtilis DSM 32540 is present in said feed or foodstuff in an amount sufficient to reduce pathogenic bacterial growth in an animal gut by at least 0.5 log compared to the same feed or foodstuff in the absence of the mutant of Bacillus subtilis DSM 32540.
A feed or foodstuff comprising fermentation products that comprise B. subtilis DSM 32540 and
- wherein, when the feed or foodstuff is fed to an animal, it inhibits the growth of at least one of:
- Clostridium perfringens, Clostridium difficile, Enterococcus cecorum, Streptococcus gallinaceus and Streptococcus suis bacteria compared to growth when the feed or foodstuff is without said Bacillus subtilis DSM 32540;
- and
- wherein the Bacillus subtilis DSM 32540 is present in the feed or foodstuff in an amount sufficient to reduce pathogenic bacterial growth in an animal gut by at least 0.5 log when fed to said animal.
Dependencies are inferred from the claim text.