MRNA-loaded lipid nanoparticles and processes of making the same

A lipid nanoparticle for the delivery of mRNA to a subject in need thereof, the lipid nanoparticle comprising

• one or more cationic lipids at a concentration between 30 molar percent and 60 molar percent,
• one or more PEG-modified lipids at a concentration between 1 molar percent and 15 molar percent,
• one or more helper lipids comprising 1,2-Dierucoyl-sn-glycero-3-phosphoethanolamine (DEPE), and wherein the DEPE is present at a concentration between 25 molar percent and 35 molar percent, and
• one or more sterols at a concentration between 20 molar percent and 30 molar percent,
• wherein the molar percents are of total lipids in the lipid nanoparticle and wherein the lipid nanoparticle encapsulates the mRNA.