Biopesticide formulation including Plicosepalus acaciae, Ocimum basilicum, and Eucalyptus tereticornis extracts

Abstract

A biopesticide formulation is provided, including extracts of Plicosepalus acacia, Ocimum basilicum, and Eucalyptus tereticornis. The biopesticide formulation may include a 3:4:4 ratio of Plicosepalus acaciae:Ocimum basilicum:Eucalyptus tereticornis. The biopesticide formulation may be formulated as a nano-emulsion and may have an LD50 of 4.57 μg/larva and a LC50 of 247 μg/mL against Rhynchophorus ferrugineus. The biopesticide formulation may be made by a method including extracting Plicosepalus acaciae powder, Eucalyptus tereticornis callus, and Ocimum basilicum callus using methanol, drying the extracts, and mixing the dried extracts. The biopesticide formulation may then be formulated as a nano-emulsion by vigorous mixing with a surfactant and a co-surfactant.

Dependencies are inferred from the claim text.