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US 12,693,277 B2Granted
Systems and methods of pH modeling and control
AssigneeRegeneron Pharmaceuticals, Inc.
InventorRoss Browne
Life of this patent
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Abstract
The disclosure relates to systems and methods for controlling the pH of a sample, comprising measuring an initial pH, adding an amount of titrant and measuring a second pH, and using non-dimensional modeling to normalize titrant and determine the amount of titrant needed to reach a final pH. The systems and methods can be used to control pH during viral inactivation or titration of protein samples.
Claims39
A method comprising:
- generating a reference titration curve from a reference sample;
- generating a model based on a regression of the reference titration curve;
- measuring an initial pH (pHinitial) of a sample;
- adding at least a first amount of titrant (Titrantn) to the sample and measuring at least a first additional pH value (pHn), Titrantn being an amount of titrant added to the sample to reach pHn, wherein pHn is different from pHinitial, and wherein the initial pH (pHinitial) and the first additional pH value (pHn) are measured using a pH probe or a pH meter of a pH flow cell;
- applying the model to determine a second amount of titrant (Titrantn+1) to reach a target pH based on pHinitial, Titrantn, and pHn, wherein the target pH is a first intermediate target pH;
- and
- based upon the result obtained by the model for determining a second amount of titrant Titrantn+i), adding the second amount of titrant (Titrantn+1) to the sample.
4 more elements
Dependencies are inferred from the claim text.