Platform for native liquid chromatography-mass spectrometry
AssigneeRegeneron Pharmaceuticals, Inc.
InventorsShunhai Wang, Yuetian Yan
Life of this patent
Estimating the term…
Abstract
Disclosed are native liquid chromatography-mass spectrometry systems and methods of use. A native liquid chromatography-mass spectrometry system can include a liquid chromatography system capable of separating a sample; and an electrospray ionization mass spectrometry (ESI-MS) system in fluid communication with the liquid chromatography system, wherein the ESI-MS system comprises a multi-nozzle electrospray ionization emitter and a system for modifying a desolvation gas and a mass spectrometer, wherein the mass spectrometer is configured to receive ions and characterize mass to charge ratio of ions.
Claims19
A native liquid chromatography-mass spectrometry system, comprising:
- a liquid chromatography system capable of separating a sample;
- and
- an electrospray ionization mass spectrometry (ESI-MS) system in fluid communication with the liquid chromatography system, wherein the ESI-MS system comprises a multi-nozzle electrospray ionization emitter, a system for modifying a desolvation gas, wherein the desolvation gas is modified with about 5% ammonia in acetonitrile, and a mass spectrometer, wherein the mass spectrometer is configured to receive ions and characterize mass to charge ratio of ions.
A method of characterizing a non-reduced intact protein in a sample, comprising:
- supplying the sample to a native liquid chromatography system capable of sample separation and fragmentation to obtain a fragment sample, the liquid chromatography system comprising a size exclusion chromatography system (SEC) column or an ion exchange chromatography (IEX) column;
- analyzing the fragmented sample by use of an electrospray ionization mass spectrometry (ESI-MS) system in fluid communication with the liquid chromatography system, wherein the ESI-MS system comprises a multi-nozzle electrospray ionization emitter, a system for modifying a desolvation gas, wherein the desolvation gas is modified with about 5% ammonia in acetonitrile, and a mass spectrometer, wherein the mass spectrometer is configured to receive ions and characterize mass to charge ratio of ions to identify components of the protein to characterize the protein;
- and
- wherein the method does not require deglycosylation of the protein in the sample prior to supplying the sample to the liquid chromatography system.
Dependencies are inferred from the claim text.