Method for localizing or tracking emitters in a sample

A light microscope for localizing or tracking emitters in a sample, comprising

• a light source which is configured to generate illumination light which induces or modulates light emissions from emitters in the sample,
• a light modulator which is configured to generate an intensity distribution of the illumination light comprising a local minimum in the sample,
• a scanning device which is configured to move the intensity distribution of the illumination light and the sample relative to one another in a measuring scanning movement,
• a control unit which is configured to control the scanning device,
• a detector which is configured to detect light emissions of a measuring emitter induced or modulated by the illumination light,
• a computing unit which is configured to estimate a position of the measuring emitter based on the detected light emissions and the positions of the local minimum of the intensity distribution assigned to the light emissions,
• wherein the computing unit is configured to correct the estimated position of the measurement emitter based on calibration data dependent on a speed and/or an acceleration of the measurement scanning movement, and/or in that the control unit is configured to adapt an actuation signal of the scanning device based on calibration data dependent on a speed and/or an acceleration of the measurement scanning movement, wherein the calibration data comprise localization data of a calibration emitter obtained by means of at least one calibration scanning movement of the scanning device.