US 12,692,627 B2 · Method of identifying intracellular secretory protein or tissue-specific secretory protein

Abstract

The present invention relates to a method of identifying an intracellular secretory protein or tissue-specific secretory protein, by using a proximity labeling system. When the method according to the present invention is used, it is possible to clearly identify an intracellular secretory protein and to dynamically track the spatiotemporal dynamics of a secretory protein secreted from a specific tissue in a living subject such that it can be effectively utilized for the research on endocrine signals between tissues, and particularly, since it can be applied in situ, the scope of application can be further expanded. Therefore, the present invention can be applied to various disease models or tissues to discover new biomarkers and therapeutic target proteins associated with diseases.

Claims12

Dependent claims

A method for identifying an intracellular secretory protein or tissue-specific secretory protein, comprising the steps of:

(a) expressing a fusion protein in cells or expressing the fusion protein tissue-specifically in a subject, wherein the fusion protein is a fusion protein in which an ER lumen targeting membrane protein and a biotin ligase are fused, wherein the biotin ligase comprises at least one selected from the group consisting of BioID and TurboID;
(b) obtaining a biotinylated protein or peptide from a sample of the cells or the subject;
and
(c) analyzing the protein or peptide to identify a secretory protein or peptide.

1 more element

Dependencies are inferred from the claim text.

Facts

Application

17/682,430Filed 28 Feb 2022

Priority

KR 10-2021-003494117 Mar 2021

Published

US 2022/0298513 A122 Sep 2022

Granted

28 Jul 2026

Term ends

Not estimatedThe source records don’t support an estimate.

Classification12 codes

C40B 30/06
Methods of screening libraries: by measuring effects on living organisms, tissues or cells
G01N 33/5035
For testing non-proliferative effects: on sub-cellular localization
C12N 9/93
Enzymes; Proenzymes; Compositions thereof ; Processes for preparing, activating, inhibiting, separating or purifying enzymes: ligases
C12Y 603/04015
Other carbon-nitrogen ligases: biotin-[acetyl-CoA-carboxylase] ligase

Method of identifying intracellular secretory protein or tissue-specific secretory protein

Life of this patent

Method of identifying intracellular secretory protein or tissue-specific secretory protein

Life of this patent

Abstract

Claims12