Glucuronosyltransferase, gene encoding same and method for using the same
AssigneesOsaka University, National Agriculture and Food Research Organization
InventorsToshiya Muranaka, Hikaru Seki, Soo Yeon Chung, Masao Ishimoto, Susumu Hiraga, Yukiko Satou
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Abstract
Glucuronosyltransferase 1 gene which catalyzes glucuronic acid transfer to the hydroxyl group at the 3-position in an oleanane-type triterpenoid is identified. Glucuronosyltransferase 1 gene having a desired activity, derived from a Fabaceae plant (soybean, Glycyrrhiza, and Lotus japonicus), and containing nucleotide sequences represented by SEQ ID Nos: 2, 4, and 6, respectively, is provided.
Claims3
A recombinant cell for producing glycyrrhizin, wherein the recombinant cell is obtained from a non-animal, capable of biologically synthesizing β-amyrin, and comprises all of the following polynucleotides (1) to (4):
- (1) a polynucleotide encoding a polypeptide having an activity of oxidizing the 11-position in an oleanane-type triterpenoid and consisting of an amino acid sequence of the following (a) or (b):
- (a) the amino acid sequence of SEQ ID NO:7, or
- (b) an amino acid sequence having at least 90% sequence identity with the amino acid sequence of SEQ ID NO:7;
- (2) a polynucleotide encoding a polypeptide having an activity of oxidizing the 30-position in an oleanane-type triterpenoid and consisting of an amino acid sequence of the following (c) or (d):
- (c) the amino acid sequence of SEQ ID NOs:
- 9, 11, or 13, or
- (d) an amino acid sequence having at least 90% sequence identity with the amino acid sequence of SEQ ID NOs:
- 9, 11, or 13;
- (3) a polynucleotide encoding a polypeptide having an activity to transfer glucuronic acid to the hydroxy group at the 2-position of glucuronic acid in an oleanane-type triterpenoid monoglucuronide and consisting of an amino acid sequence of the following (e) or (f):
- (e) the amino acid sequence of SEQ ID NO:15, or
- (f) an amino sequence having at least 90% sequence identity with the amino acid sequence of SEQ ID NO:
- 15;
- and
- (4) a polynucleotide encoding a polypeptide having an activity to transfer glucuronic acid to the hydroxy group at the 3-position in an oleanane-type triterpenoid, and consisting of an amino acid sequence of the following (g) or (h):
- (g) the amino acid sequence of SEQ ID NOs:
- 3 or 5, or
- (h) an amino acid sequence polypeptide having at least 95% sequence identity with the amino acid sequence of SEQ ID NOs:
- 3 or 5.
Dependencies are inferred from the claim text.