Method of producing L-amino acid

A method of producing an L-amino acid, comprising:

• cultivating a coryneform bacterium having L-amino acid-producing ability in a culture medium to accumulate an L-amino acid in the culture medium and/or in cells of the bacterium;
• and
• collecting the L-amino acid from the culture medium and/or the cells;
• wherein the L-amino acid is L-glutamic acid family, and
• wherein the bacterium has a modification for increasing activity of acetate kinase, and optionally an additional a modification for increasing activity of fructose-1,6-bisphosphatase, combinations thereof;
• wherein expression of the gene encoding acetate kinase is increased by increasing the copy number of the gene and/or by modifying an expression regulatory sequence of the gene;
• wherein the acetate kinase is selected from the group consisting of:
• (i) a protein comprising the amino acid sequence of SEQ ID NO:
• 8 or 10,
• (ii) a protein comprising the amino acid sequence of SEQ ID NO:
• 8 or 10 except that said amino acid sequence includes substitution, deletion, insertion, and/or addition of 1 to 10 amino acid residues, and has acetate kinase activity, and
• (iii) a protein comprising an amino acid sequence having 90% or more identity to the amino acid sequence of SEQ ID NO:
• 8 or 10, and has acetate kinase activity;
• wherein the fructose-1,6-bisphosphatase is selected from the group consisting of:
• (i) a protein comprising the amino acid sequence of SEQ ID NO:
• 12 or 14,
• (ii) a protein comprising the amino acid sequence of SEQ ID NO:
• 12 or 14 except that said amino acid sequence includes substitution, deletion, insertion, and/or addition of 1 to 10 amino acid residues, and has fructose-1,6-bisphosphatase activity, and
• (iii) a protein comprising an amino acid sequence having 90% or more identity to the amino acid sequence of SEQ ID NO:
• 12 or 14, and has fructose-1,6-bisphosphatase activity;
• wherein the bacterium has been further modified so that the activity of phosphoketolase is increased as compared to an unmodified bacterium;
• and
• wherein the bacterium is of the genus Corynebacterium.