Recombinant strain producing L-lysine and construction methods therefor and use thereof

A method for producing L-lysine, comprising culturing a first recombinant strain contains a polynucleotide encoding a polypeptide that comprise an amino acid sequence of SEQ ID NO:

• 3, wherein the lysine residue at position 176 is substituted with an asparagine residue;
• the polynucleotide sequence is formed by mutation of the base at position 528 of the polynucleotide sequence shown in SEQ ID NO:
• 1;
• and the mutation includes base mutation of adenine (A) to cytosine (C) at position 528 of the polynucleotide sequence shown in SEQ ID NO:
• 1;
• or a second recombinant strain contains the promoter nucleotide sequence comprises the nucleotide sequence obtained by mutating the bases at positions −49, −51 and −54 to −58 in the promoter region shown in SEQ ID NO:
• 29;
• or an expression cassette comprising the promoter nucleotide sequence comprises the nucleotide sequence obtained by mutating the bases at positions −49, −51 and −54 to −58 in the promoter region shown in SEQ ID NO:
• 29;
• or a second recombinant vector comprises the promoter nucleotide sequence comprises the nucleotide sequence obtained by mutating the bases at positions −49, −51 and −54 to −58 in the promoter region shown in SEQ ID NO:
• 29;
• wherein in the second recombinant strain or in the second recombinant vector or in the expression cassette, the nucleotide at position −49 of the promoter region shown in SEQ ID NO:
• 29 is mutated from cytosine (C) to adenine (A), the nucleotide at position −51 is mutated from guanine (G) to thymine (T), and the nucleotide sequence from positions −54 to −58 is mutated from CTGCA to GGTGT;
• and
• both the first recombinant strain and the second recombinant strain use Corynebacterium glutamicum YP97158 as the host strain, and recovering L-lysine from the culture.