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US 12,692,502 B2Granted
Biological devices and methods of use thereof to produce steviol glycosides
AssigneeBio Capital Holdings, LLC
InventorRaul Cuero Rengifo
Life of this patent
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Abstract
Described herein are devices and methods for increasing the production of steviol glycosides, which have industrial and economic value. The steviol glycosides produced by the devices and methods disclosed herein do not require the ultra purification that is common in conventional or commercial methods and do not have a bitter aftertaste, making them better suited as flavor-enhancing additives to food, pharmaceutical, and nutritional supplement products.
Claims10
A vector comprising a DNA construct comprising the following genetic components:
- a) a gene that expresses hexokinase having SEQ ID NO:
- 2 or at least 90% homology thereto;
- b) a gene that expresses geranylgeranyl pyrophosphate synthase 2 having SEQ ID NO:
- 4 or at least 90% homology thereto;
- c) a gene that expresses 1-deoxy-D-xylulose-5-phosphate synthase having SEQ ID NO:
- 5 or at least 90% homology thereto;
- d) a gene that expresses mevalonate-5-kinase having SEQ ID NO:
- 6 or at least 90% homology thereto;
- e) a gene that expresses isopentenyl pyrophosphate isomerase having SEQ ID NO:
- 7 or at least 90% homology thereto;
- f) a gene that expresses mevalonate pyrophosphate decarboxylase having SEQ ID NO:
- 8 or at least 90% homology thereto;
- and
- g) a gene that expresses UDP-glycosyltransferase 76G1 having a His 155Leu mutation and SEQ ID NO:
- 12 or at least 90% homology thereto;
- wherein the vector is a plasmid.
13 more elements
Dependencies are inferred from the claim text.