Back to results
US 12,692,501 B2Granted
Aptamer nanofibers and kill-switches and uses thereof
AssigneesThe University of North Carolina at Charlotte, The Penn State Research Foundation
InventorsKirill Afonin, Nikolay Dokholyan
Life of this patent
Estimating the term…
Abstract
This invention relates to nanofibers comprising aptamers, which have increased stability and activity relative to free aptamers. The invention further relates to kill-switch nanofibers which disrupt the aptamer nanofibers. The invention further relates to methods of using the aptamer nanofibers and the kill-switch nanofibers to regulate the activity of extracellular targets recognized by the aptamers.
Claims15
A single strand DNA molecule comprising:
- a) a polynucleotide of about 40 to about 70 nucleotides in length, the polynucleotide comprising:
- an RNA binding sequence of about 20 to about 30 nucleotides in length;
- and
- a toehold sequence of about 10 to about 20 nucleotides length linked to the 5′ and 3′ ends of the RNA binding sequence;
- and
- b) an aptamer linked to the 5′ and/or 3′ end of the polynucleotide;
- wherein the aptamer(s) binds an extracellular target.
4 more elements
Dependencies are inferred from the claim text.