Methods of preparing oligonucleotide compositions using ultrafiltration/diafiltration
AssigneeBiogen MA Inc.
InventorsRobert S. Gronke, Jonas P. Immel-Brown, Geetha Govindan
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Abstract
Disclosed herein are methods for preparing compositions containing oligonucleotides. Methods of this disclosure involve subjecting an aqueous solution of a oligonucleotide to ultrafiltration/diafiltration (UF/DF) in order to form a retentate containing the oligonucleotide, where the ultrafiltration/diafiltration (UF/DF) is carried out using an aqueous buffer solution containing one or more salts. Also disclosed herein are oligonucleotide-containing compositions obtained by these methods.
Claims22
A method for preparing a composition comprising an oligonucleotide, the method comprising subjecting an aqueous solution of the oligonucleotide to ultrafiltration/diafiltration (UF/DF) to form a retentate comprising the oligonucleotide, wherein the ultrafiltration/diafiltration (UF/DF) is carried out using an aqueous buffer solution and wherein the aqueous buffer solution comprises i) sodium acetate and ammonium acetate or ii) sodium acetate and potassium acetate.
Dependencies are inferred from the claim text.