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US 12,692,490 B2Granted
Method for preparing RNA derived from skin surface lipids
AssigneeKao Corporation
InventorsTetsuya Kuwano, Takayoshi Inoue
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Abstract
Provided is a method for collecting RNA contained in skin surface lipids (SSL). A method for preparing SSL-derived RNA. In this method, an aqueous solution containing RNA prepared from SSL of a subject is mixed with a water-soluble organic solvent, and the resulting mixture is allowed to contact with a solid phase material to separate RNA. By adjusting the concentration of the water-soluble organic solvent in the mixture, the yield of RNA improves.
Claims5
A method for preparing RNA derived from skin surface lipids, the method comprising:
- preparing an aqueous solution comprising RNA, wherein the RNA is separated from skin surface lipids obtained from a subject;
- mixing the aqueous solution with ethanol to prepare a mixture;
- and
- allowing the mixture to contact with a silica-based solid phase material, so that the RNA in the mixture is adsorbed to the silica-based solid phase material,
- wherein a final concentration of the ethanol in the mixture before the contact with the solid phase material is 42 vol % or more and 45 vol % or less.
2 more elements
Dependencies are inferred from the claim text.