Chimeric lysozyme variant and application thereof in animal feed additive
AssigneesNanjing Bestzyme Bio-Engineering Co., Ltd., Jinan Bestzyme Bio-Engineering Co., Ltd.
InventorsFei Zheng, Ting Yan, Jidong Zhu, Hong Xu, Yan Sun, Yanjie Liu
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Abstract
A chimeric lysozyme variant has an improved heat resistance and is encoded by a polynucleotide. A novel chimeric lysozyme sequence is constructed, and high-efficiency expression in a host cell is achieved. The thermal stability of the chimeric lysozyme under the condition of 60° C. is nearly doubled compared to a parental sequence. The chimeric lysozyme variant can be used to prepare product that is antibiotic, for example, animal feed additive containing the chimeric lysozyme variant and an animal feed containing the animal feed additive.
Claims12
A chimeric lysozyme variant, wherein the chimeric lysozyme variant has an amino acid sequence set forth in SEQ ID NO:
- 8, or has at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% sequence identity to SEQ ID NO:
- 8;
- and wherein the chimeric lysozyme variant has a lysozyme activity and exhibits increased thermostability as compared to a lysozyme of SEQ ID NO:
- 4 or SEQ ID NO:
- 6.
Dependencies are inferred from the claim text.