PUMA1 polymerases and uses thereof
Assignee10x Genomics, Inc.
InventorsLorita Boghospor, Yufeng Qian
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Abstract
The present disclosure relates in some aspects to recombinant polymerases, variant PumA1 polymerases, and compositions thereof. Also provided herein are methods of using the recombinant polymerases and/or variant PumA1 polymerases for nucleic acid amplification (e.g., rolling circle amplification). In some aspects, the compositions and methods disclosed herein provide more robust amplification (e.g., RCA) reactions for improved in vitro and in situ analysis.
Claims20
A method of performing a rolling circle amplification (RCA), the method comprising contacting a biological sample containing a circular DNA to be amplified with a recombinant polymerase comprising an amino acid sequence having at least 99% sequence identity to the sequence set forth in SEQ ID NO:2, wherein the circular DNA is amplified by rolling circle amplification (RCA) using the recombinant polymerase.
Dependencies are inferred from the claim text.