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US 12,692,483 B2Granted
Protein-based purification matrices and methods of using the same
AssigneeDonaldson Company, Inc.
InventorsKelli M. Luginbuhl, Michael Dzuricky
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Abstract
Provided herein are protein-based purification matrices and methods of use thereof to purify biologics and/or to remove contaminants from a composition. Methods of bringing two or more biologics in close proximity are also provided. The disclosed compositions and methods allow for faster, more efficient purification of a biologic compared to traditional affinity chromatography.
Claims21
A method of purifying a viral particle comprising:
- (i) contacting the viral particle with a fusion protein comprising (a) at least one low-density lipoprotein receptor (LDLR) or an extracellular domain thereof;
- and (b) at least one polypeptide with phase behavior,
- wherein the fusion protein binds to the viral particle and forms a complex;
- (ii) contacting the complex with a first environmental factor;
- (iii) separating the complex from at least one contaminant;
- and
- (iv) separating the viral particle from the fusion protein by contacting the complex with a second environmental factor.
4 more elements
Dependencies are inferred from the claim text.