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US 12,692,481 B2Granted
Baculovirus expression system
AssigneeVirovek, Inc.
InventorHaifeng Chen
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Abstract
The present disclosure relates to a heterologous recombinant baculovirus (rBV) expression system for the production of foreign heterologous proteins in insect cells. This system comprises a recombinant baculovirus backbone within a genome with a deletion in the cathepsin gene into which foreign gene cassettes can be integrated, and an insect cell that can be infected by the Δv-cath-rBV, and in which the foreign proteins and/or viral vectors or particles are expressed.
Claims14
A recombinant baculovirus (rBV) genome comprising an rBV DNA backbone, wherein the rBV DNA backbone comprises:
- (i) a chitinase gene;
- (ii) a deletion of a v-cath gene, and
- (iii) a DNA fragment enabling integration of one or more protein expression cassettes into the rBV DNA backbone, wherein the one or more protein expression cassettes comprise:
- at least one gene comprising a sequence encoding an AAV capsid protein,
- an insect cell promoter operably linked to the at least one gene, and
- two DNA sequences enabling the one or more protein expression cassettes to integrate into the rBV DNA backbone;
- and
- upon infection of the rBV genome into an insect cell, AAV vectors are produced at a level about 3 times greater as compared to AAV vectors produced from an rBV DNA backbone without a deletion of the v-cath gene.
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