Medium composition for culturing porcine pluripotent stem cells

A culture medium composition for culturing porcine pluripotent stem cells, comprising:

• a. the culture medium composition comprising
• 3.03×10−4 to 6.06×10−3 μM basic fibroblast growth factor (bFGF);
• 1.50×10−4 to 5.40×10−4 μM Activin A;
• 0.5 to 2.50 μM CHIR99021 (6-[[2-[[4-(2,4-Dichlorophenyl)-5-(5-methyl-1H-imidazol-2-yl)-2-pyrimidinyl]amino]ethyl]amino]-3-pyridinecarbonitrile);
• and
• 1.20 to 3.00 μM IWR-1 (4-(1,3,3a,4,7,7a-Hexahydro-1,3-dioxo-4,7-methano-2H-isoindol-2-yl)-N-8-quinolinyl-Benzamide), and
• b. porcine pluripotent stem cells,
• wherein the culture medium composition is free of fetal bovine serum and the culture medium composition is capable of maintaining porcine pluripotent stem cells in an undifferentiated state and allowing for their long-term cryopreservation, and
• wherein the culture medium composition has the ability to maintain the pluripotent stem cells in an undifferentiated state with in vivo differentiation capacity, and the porcine pluripotent stem cells cultured in the culture medium composition form teratomas and/or chimeras.