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US 12,692,476 B2Granted
Compositions and methods for embryonic stem cell expansion
AssigneeLineage Cell Therapeutics, Inc.
InventorsLilach Alon, Rami Skaliter, Ravid Tikotzki, Dana Hayoun Neeman, Ofer Wiser
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Abstract
Provided herein are methods and compositions for expansion of human embryonic stem with suspendable expansion complexes including microcarriers.
Claims18
A method for expanding and maintaining human stem cells in an undifferentiated, pluripotent state, the method comprising the steps of (a) simultaneously combining human stem cells, human laminin 511 E8 fragment, and polystyrene microcarriers in growth medium to form a suspendable expansion complex, and (b) culturing the suspendable expansion complex for a period of time, thereby producing cultured cells.
Dependencies are inferred from the claim text.