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US 12,692,475 B2Granted
Microcarrier for cell culture and method for preparing the same
AssigneeLG Chem, Ltd.
InventorsMinchae Kim, Yeji Kim, Jee Seon Kim
Life of this patent
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Abstract
The present application relates to microcarrier particles for cell culture, a method for preparing the particles, and a cell culture medium composition including the particles. According to the present application, a microcarrier having a high degree of uniformity in shape or form, having porosity, and advantageous for cell attachment and isolation of cultured cells is provided.
Claims15
A method for preparing microcarrier particles for cell culture, the method comprising the steps of:
- mixing a continuous phase composition (A) satisfying the following conditions 1 and 2;
- and a dispersive phase composition (B) containing a polymerizable monomer (b1) and then performing suspension polymerization,
- wherein the dispersive phase composition (B) comprises a hydrocarbon oil (b3) in an amount of 10 to 20% by weight based on the total content of the dispersive phase composition, wherein the microcarrier particles include microcarrier particles having a density in the range of 0.95 g/cm3 to 1.00 g/cm3:
- 45 mN/m<Surface Tension of Continuous Phase Composition≤54 mN/m [Condition 1]
- Viscosity of Continuous Phase Composition≥2.0 cp, and [Condition 2]
- wherein, in the [Condition 1], the surface tension is measured at a normal temperature according to a ring method, and in the [Condition 2], the viscosity is measured under conditions of a shear rate in the range of 66 to 264 l/s and a normal temperature.
3 more elements
Dependencies are inferred from the claim text.