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US 12,692,467 B2Granted
High-throughput magnetic actuation platform for cancer treatment screening
AssigneePurdue Research Foundation
InventorsHyowon Lee, Hyunsu Park, Luis Solorio, Angel Enriquez, Sarah Libring
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Abstract
Cell-culture platforms are described that can impart cyclic strain on a biologically-relevant culture substrate. Accordingly, systems and methods of the invention can provide a more accurate model of physiological strain and forces than earlier technologies. Systems of the invention enable high-throughput experimentation in standard culturing equipment, while utilizing a 3D physiologically-relevant environment.
Claims4
A method for modeling mechanical forces on a cell culture, the method comprising:
- providing a cell-culture platform comprising a magnet, a magnetically actuated cantilever, a non-magnetic frame, and an extracellular matrix (ECM) protein for culturing cells upon, wherein a first point of the ECM protein is directly contacting a fixed portion of a frame of the cell-culture platform and a second point of the ECM protein is directly contacting a magnetically actuated portion of a cantilever that is operably coupled to the cell-culture platform, wherein the cell-culture platform does not include any other deformable membrane supporting the ECM protein, wherein the ECM protein is at least one ECM protein selected from the group consisting of fibronectin, elastin, laminin, and vitronectin, and wherein the non-magnetic frame surrounds the magnetically actuated cantilever and the magnet is positioned within the magnetically actuated cantilever;
- culturing cells on the ECM protein;
- and
- cyclically changing the distance between the fixed portion and the actuated portion to impart strain on the ECM protein and cells cultured thereon.
1 more element
Dependencies are inferred from the claim text.