A nucleic acid extraction microfluidic chip, comprising:
- a channel plate, comprising:
- a first segment, a second segment, and a third segment arranged and connected sequentially in a first direction, wherein lengths in a second direction of the first segment, the second segment, and the third segment decrease sequentially, and the first segment comprises a first sub-segment and a second sub-segment protruding in the first direction relative to the second sub-segment, and a third sub-segment between the first sub-segment and the second sub-segment;
- a mixed lysis zone located in the second segment and the third segment, wherein the mixed lysis zone comprises:
- a first liquid inlet groove, a second liquid inlet groove, and a third liquid inlet groove;
- a first channel, a second channel, and a third channel communicating with the first liquid inlet groove, the second liquid inlet groove, and the third liquid inlet groove, respectively;
- and
- a mixed lysis groove, located at the second channel and communicating with the second channel;
- an extraction zone located in the third sub-segment, wherein the extraction zone comprises:
- an extraction trough with a liquid inlet end and a liquid outlet end, and the extraction trough communicates with the first channel, the second channel, and the third channel via the liquid inlet end;
- a first gas-pressure driven port located in the first sub-segment, wherein an end of the first gas-pressure driven port communicates with an exterior, and another end of the first gas-pressure driven port communicates with the extraction zone via a first sub-channel and the liquid outlet end;
- and
- a second gas-pressure driven port located in the second sub-segment, wherein an end of the second gas-pressure driven port communicates with the exterior, and another end of the second gas-pressure driven port communicates with the extraction zone via a second sub-channel and the liquid outlet end;
- a cover plate, disposed opposite to the channel plate, wherein the cover plate comprises:
- a sample inlet at a position corresponding to the mixed lysis groove;
- and
- a first liquid inlet through hole at a position corresponding to the first liquid inlet groove, a second liquid inlet through hole at a position corresponding to the second liquid inlet groove, and a third liquid inlet through hole at a position corresponding to the third liquid inlet groove;
- and
- a solution accommodating cavity, on a side of the cover plate away from the channel plate, wherein the solution accommodating cavity comprises:
- a flushing liquid accommodating cavity, in communication with the first liquid inlet groove of the channel plate through a first through hole;
- a lysis solution accommodating cavity, in communication with the second liquid inlet groove of the channel plate through a second through hole;
- and
- an eluent accommodating cavity, in communication with the third liquid inlet groove of the channel plate through a third through hole.