Microcarrier filter bag assemblies and methods of use
AssigneeLife Technologies Corporation
InventorCharles R. Rhodes
Life of this patent
Estimating the term…
Abstract
A filter bag assembly includes: a flexible first sheet; a flexible second sheet overlying and secured to the first sheet so that a compartment is formed therebetween; a porous filter sheet disposed between the first sheet and the second sheet so as to divide the compartment into a first compartment and a second compartment; a first port secured to the second sheet so as to communicate directly with the first compartment; and a second port secured to the second sheet so as to communicate directly with the second compartment, the second port being spaced apart from the first port. The porous filter sheet is disposed so that fluid entering the first compartment through the first port must pass through the filter sheet before entering the second compartment or exiting through the second port.
Claims12
A method for filtering microcarrier beads from a liquid solution comprising cells, the method comprising:
- delivering the liquid solution with the microcarrier beads into a main compartment of a filter bag assembly, the filter bag assembly comprising:
- a flexible bag bounding the main compartment;
- a filter sheet disposed within the main compartment of the flexible bag and dividing the main compartment into a first compartment and a second compartment, the filter sheet at least partially secured within the flexible bag by a seal line upwardly spaced from a bottom edge of the flexible bag;
- receiving the liquid solution in the first compartment through an inlet port coupled to the first compartment, the inlet port further coupled to a bioreactor through a first fluid line;
- passing the liquid solution from the first compartment to the second compartment through the filter sheet;
- and
- collecting the liquid solution without the microcarrier beads through an outlet port coupled to the second compartment of the filter bag assembly, the microcarrier beads configured to be stopped by the seal line.
Dependencies are inferred from the claim text.