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US 12,691,183 B2Granted
Methods of making and using nanoparticles for treatment of bacterial biofilm
AssigneeBrown University
InventorsAnita Shukla, Yingying Wang
Life of this patent
Estimating the term…
Abstract
A method of making and using nanoparticles that target bacterial biofilm are provided.
Claims7
A method of treating a bacterial infection within a subject in need thereof, wherein the bacterial infection is characterized by a bacterial biofilm comprising
- administering to the subject a nanoparticle comprising
- (1) a core comprising gelatin and an antibiotic,
- (2) an inner first layer comprising chitosan surrounding the core and
- (3) an outer layer comprising hyaluronic acid surrounding the inner first layer at least one layer surrounding the core,
- wherein, after administration, the nanoparticle contacts the bacterial biofilm,
- wherein the hyaluronic acid is responsive to hyaluronidase in the bacterial biofilm which degrades the hyaluronic acid to expose the chitosan,
- wherein the chitosan is responsive to the pH of the bacterial biofilm, which swells the chitosan thereby allowing gelatinase in the bacterial biofilm to contact the gelatin, and
- wherein gelatinase in the bacterial biofilm degrades the gelatin of the core to release the antibiotic, to kill the bacteria.
5 more elements
A method of delivering an antibacterial to a bacterial infection within a subject in need thereof, wherein the bacterial infection is characterized by a bacterial biofilm comprising
- administering to the subject a pharmaceutical composition comprising a nanoparticle in a pharmaceutically acceptable carrier, wherein the nanoparticle comprises
- (1) a core comprising gelatin and an antibacterial,
- (2) an inner first layer comprising chitosan surrounding the core and
- (3) an outer layer comprising hyaluronic acid surrounding the inner first layer at least one layer surrounding the core,
- wherein, after administration, the nanoparticle contacts the bacterial biofilm,
- wherein the hyaluronic acid is responsive to hyaluronidase in the bacterial biofilm which degrades the hyaluronic acid to reveal the chitosan,
- wherein the chitosan is responsive to the pH of the bacterial biofilm which swells the chitosan thereby allowing gelatinase in the bacterial biofilm to contact the gelatin, and
- wherein gelatinase in the bacterial biofilm degrades the gelatin of the core to release the antibacterial, such that the antibacterial contacts bacteria within the bacterial biofilm.
5 more elements
A method of treating a bacterial biofilm within an individual in need thereof comprising
- administering to the subject a nanoparticle comprising
- (1) a core comprising gelatin and an antibiotic
- (2) an inner first layer comprising chitosan surrounding the core, and
- (3) an outer layer comprising hyaluronic acid surrounding the inner layer
- wherein, after administration, the nanoparticle contacts the bacterial biofilm,
- wherein the hyaluronic acid is responsive to hyaluronidase in the bacterial biofilm which degrades the hyaluronic acid revealing the inner first layer comprising chitosan,
- wherein the chitosan is responsive to the pH of the bacterial biofilm which swells the chitosan thereby allowing gelatinase in the bacterial biofilm to contact the gelatin, and
- wherein the gelatinase in the bacterial biofilm degrades the gelatin of the core to release the antibiotic, thereby treating the bacterial biofilm.
5 more elements
Dependencies are inferred from the claim text.