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AssigneeThe United States of America as represented by the Secretary of Agriculture
InventorsDavid L. Suarez, Mary J. Pantin Jackwood, Sungsu Youk
Estimating the term…
The disclosure relates to avian influenza vaccines produced through reverse genetics to protect from A/Turkey/Indiana/22-like virus, methods for producing such vaccines, and method for using such vaccines to protect poultry. The disclosure also relates to methods of producing influenza viruses comprising chimeric polynucleotides having an HA non-coding sequence from an apathogenic high growth influenza virus and a polynucleotide encoding an HA protein from a highly pathogenic avian influenza virus or variant thereof, and/or having a chimeric polynucleotide having an NA non-coding sequence from an apathogenic high growth influenza virus and a polynucleotide encoding an NA protein from a highly pathogenic avian influenza virus or variant thereof.
A chimeric polynucleotide comprising:
A rescued influenza virus comprising polynucleotides encoding M1/M2, NP, PB1, PA, PB2, and NS1/NS2 from an PR8 apathogenic high growth influenza A virus, a polynucleotide encoding an attenuated HA from a A/turkey/Indiana/22-003707-003/2022 high highly pathogenic avian influenza virus or a variant thereof having 96% identity thereto, and a polynucleotide encoding an N1, N2, N3, N4, N5, N6, N7, N8, or N9 NA;
A method of producing an influenza virus, the method comprising introducing into a population of host cells a plurality of vectors comprising polynucleotides corresponding to at least six internal segments of an PR8 apathogenic high growth influenza virus, a chimeric polynucleotide comprising an HA non-coding sequence from an PR8 apathogenic high growth influenza virus and a polynucleotide encoding an attenuated HA protein from a A/turkey/Indiana/22-003707-003/2022 highly pathogenic avian influenza virus or a variant thereof having 96% identity thereto, and a chimeric polynucleotide comprising an NA non-coding sequence from an PR8 apathogenic high growth influenza virus and a polynucleotide encoding an N1, N2, N3, N4, N5, N6, N7, N8, or N9 NA protein or a variant thereof having 96% identity thereto, culturing the population of host cells at a temperature that is less or equal to about 35° C., and recovering the virus;
Dependencies are inferred from the claim text.