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US 12,690,596 B2Granted
Methods and compositions for microbial delivery of double stranded RNA
AssigneeRnaissance AG LLC
InventorsJohn L. Killmer, Anil Kumar
Life of this patent
Estimating the term…
Abstract
Methods and materials for improved delivery of dsRNA are presented. In particular, methods for controlling an insect pest by stably delivering large quantities of dsRNA to the insect pest are provided. Compositions comprising a bacterium containing a large quantity of dsRNA targeting an insect gene in which the dsRNA exhibits increased stability and activity are also provided.
Claims12
A method for controlling an insect pest, the method comprising:
- contacting the insect pest in a body of water or insect saliva with a composition comprising a microorganism comprising:
- (i) a promoter operably linked to a DNA sequence encoding a dsRNA molecule that specifically inhibits expression of a target gene in an insect pest, wherein the target gene is selected from extracellular signal-regulated kinase 1 (erkA), tubulin, vATPase, acetyl choline esterase, chitin synthase gene A, beta-actin, and genes coding for inhibitors of apoptosis;
- and
- (ii) a promoter operably linked to a DNA sequence comprising a coat protein gene encoding a capsid protein or N-terminus of the capsid protein coding sequence (CDS);
- and wherein the dsRNA in the composition comprising the microorganism exhibits at least 60% undegraded dsRNA for at least 7 days in the body of water or insect saliva relative to the dsRNA when isolated.
2 more elements
Dependencies are inferred from the claim text.