Viable cell compositions, and methods related to same
AssigneeGallant Pet, Inc.
InventorsShelly J. Zacharias, Erik J. Woods
Life of this patent
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Abstract
Disclosed are viable cell compositions and related methods of preparation, maintenance and use. The viable cell composition can contain specified levels of cells, hydroxyethyl starch, and dimethylsulfoxide, and can be cryopreserved. The cryopreserved form of the composition can be thawed and combined with an aqueous liquid diluting medium to prepare a diluted viable cell composition that can contain specified, reduced levels of the dimethylsulfoxide and hydroxyethyl starch. The diluting medium can contain trehalose. The prepared, diluted viable cell composition can be administered to a patient.
Claims20
A method for treating a patient with viable uterine regenerative cells, comprising:
- thawing a cryopreserved composition comprising viable uterine regenerative cells and a saline cryopreservation medium, wherein the saline cryopreservation medium of the cryopreserved composition consists of dimethylsulfoxide at a concentration of 2% and hydroxyethyl starch at a concentration of about 2% to about 10% producing a thawed viable uterine regenerative cell suspension;
- combining an aqueous liquid diluting medium with the thawed viable uterine regenerative cell suspension in a volume ratio of at least 3:1 to produce a diluted viable uterine regenerative cell composition containing hydroxyethyl starch and dimethylsulfoxide, wherein the dimethylsulfoxide is present in the diluted viable uterine regenerative cell composition at a concentration of about 0.5% or less;
- and
- administering the diluted viable uterine regenerative cell composition intravenously to the patient.
Dependencies are inferred from the claim text.