Method for capturing and identifying cellular agglutinates for detecting multiplex anti-erythrocyte antibodies

An in vitro method for detecting anti-erythrocyte antibodies in a sample, comprising at least the following steps:

• a) bringing said sample containing anti-erythrocyte antibodies into contact with one or more test erythrocyte(s) or a suspension of test erythrocyte(s) of known phenotype, under conditions capable of inducing hemagglutination, so as to obtain a reaction mixture;
• b) bringing said reaction mixture into contact with a solid support containing a plurality of defined adsorption zones which have previously bonded antibodies or antibody fragments capable of binding antigenic determinants present on said test erythrocyte(s), wherein at least two of the adsorption zones each bond a plurality of antibodies and/or antibody fragment(s) capable of binding a distinct erythrocyte antigenic determinant;
• c) determining the presence or absence of a hemagglutination reaction on at least one of said adsorption zones, wherein the presence of a hemagglutination reaction indicates the presence of anti-erythrocyte antibodies from the sample which recognize the same antigenic determinants recognized by the previously bonded antibodies or antibody fragments and wherein the absence of a hemagglutination reaction indicates the absence of anti-erythrocyte antibodies from the sample which recognize the same antigenic determinants recognized by the previously bonded antibodies or antibody fragments;
• said steps a) and b) being carried out separately or simultaneously.