Fluorescent protein sensor capable of quantitatively measuring oxidation degree of methionine residues of specific protein, and use thereof

Abstract

A fluorescent protein sensor capable of quantitatively measuring oxidation degree of methionine residues of a specific protein, and a use thereof. Specifically, a fluorescent biosensor recombinant protein in which an MsrB protein, a cpYFP protein, a thioredoxin 3 protein, a linker protein and a G protein are linked in this order, and which is capable of quantitatively measuring the oxidation degree of methionine residues of a target protein; a fluorescent biosensor comprising same; a method for measuring oxidation degree of methionine residues of a target protein; and an information providing method for diagnosis of oxidative stress-associated diseases; and a method for screening for a therapeutic agent for oxidative stress-associated diseases. A recombinant protein for a fluorescent biosensor for accurately and quantitatively measuring, through the detection of a specific change in fluorescence, oxidation degree of methionine residues of a specific protein, but not all proteins, even in a mixed biological sample.

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