Nanoflower immunochromatographic strip for detecting heavy metal mercury ions and use thereof

Abstract

A nanoflower immunochromatographic strip for detecting heavy metal mercury ions is provided. The nanoflower immunochromatographic strip includes the following components: a plastic outer shell, a sample pad, an immunoprobe joint pad, a nitrocellulose membrane and an absorbent pad; the immunoprobe joint pad is dropwise added with a gold nano-immunoprobe labeled with an anti-mercury-ion monoclonal antibody; the anti-mercury-ion monoclonal antibody is a monoclonal antibody secreted by an anti-Hg2+-ITCBE hapten murine hybridoma cell strain 7A1; and a preservation number of the murine hybridoma cell strain 7A1 is CGMCC No. 23879. The nanoflower immunochromatographic strip detects a heavy metal hapten Hg2+-ITCBE, with a detection threshold of 50 ng/mL and a detection limit of 0.39 ng/mL, has strong specificity, high sensitivity, good repeatability and rapid detection, and is of great significance for monitoring residue of heavy metal mercury.

Dependencies are inferred from the claim text.