Magnetic immunosensor with trench configuration and method of use

A point-of-care magnetic immunosensing device, comprising:

• a cartridge housing comprising a base;
• a sample chamber comprising a sample inlet for receiving a sample;
• a conduit in fluidic communication with the sample chamber;
• a sensor chip located within the conduit and formed on the base of the cartridge housing, wherein the sensor chip comprises a single sensing electrode comprising:
• a) a silicon wafer, b) a single trench formed in the silicon wafer;
• a dissolvable dry matrix comprising magnetically susceptible beads, wherein the matrix is configured to dissolve in the sample fluid;
• and
• a permanent high-field magnet;
• and
• a pump for moving the sample from the sample chamber through the conduit to the sensing electrode,
• wherein the single trench comprises a two-dimensional array of microfabricated electrodes comprising a line of microelectrodes.