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AssigneeOil Crops Research Institute, Chinese Academy of Agricultural Sciences
InventorsQi Zhang, Yizhen Bai, Nanri Yin, Fei Ma, Peiwu Li
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An immunoadsorbent and a composite affinity column for purifying fumonisin B1, anguidin, T-2 toxin, zearalenone, and vomitoxin. The immunoadsorbent includes a solid phase carrier, and a fumonisin B1 monoclonal antibody, an anguidin monoclonal antibody, a T-2 toxin monoclonal antibody, a zearalenone monoclonal antibody and a vomitoxin monoclonal antibody which are coupled to the solid phase carrier, the anguidin monoclonal antibody is a monoclonal antibody secreted by a hybridoma cell strain DAS5G11E7 having an accession number of CCTCCNO:C201881. The affinity column can be used for high performance liquid chromatography-mass spectrometry detection of the fumonisin B1, the anguidin, the T-2 toxin, the zearalenone and the vomitoxin, and has stable performance. Furthermore, an economical, quick, precise and safe detection method is established of the basis of the affinity column, and can be used for purifying and detecting samples of the five toxins without mutual interference and influence.
An immunoadsorbent for purifying fumonisin B1, anguidin, T-2 toxin, zearalenone, and vomitoxin, the immunoadsorbent comprises a solid phase carrier, and an anti-fumonisin B1 monoclonal antibody, an anti-anguidin monoclonal antibody, an anti-T-2 toxin monoclonal antibody, an anti-zearalenone monoclonal antibody, and an anti-vomitoxin monoclonal antibody which are coupled to the solid phase carrier, and the anti-anguidin monoclonal antibody is a monoclonal antibody secreted by a hybridoma cell strain DAS5G11E7 having an accession number of CCTCCNO:C201881.
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