Single cell selection and isolation
AssigneeErasmus University Medical Center Rotterdam
InventorMiao-Ping Chien
Life of this patent
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Abstract
According to one method, a sample with cells contains a phototagging agent. The sample is imaged to identify at least one target cell to be isolated. The identified target cell in the sample is selectively irradiated with photo-activating light for selectively activating the phototagging agent in the target cell to change its fluorescence response. The irradiated target cell is isolated from other cells in the sample based on a difference in its fluorescence response compared to non-activated phototagging agent in the other cells. Further aspects are directed to a corresponding microscope system and chemical compound for use as the phototagging agent.
Claims20
A method for isolating cells, the method comprising
- providing a sample with cells containing a phototagging agent comprising a caged fluorophore moiety, optionally conjugated to a photosensitizer moiety;
- imaging the sample to identify at least one target cell to be isolated;
- selectively irradiating the identified target cell in the sample with photo-activating light for selectively activating the phototagging agent in the target cell to change its fluorescence response;
- and
- isolating the irradiated target cell from other cells in the sample based on a difference in its fluorescence response compared to non-activated phototagging agent in the other cells.
Dependencies are inferred from the claim text.