Devices and methods for laser capture microdissection

A system for a biological process, comprising:

• (a) a sample carrier, comprising:
• a cap formed from a substrate with an upper portion of the substrate comprising an outer perimeter, a first shoulder, and the top surface;
• an intermediate portion of the substrate, wherein the intermediate portion comprises a first seating area with a first diameter and a second shoulder;
• a lower portion of the substrate, wherein the lower portion comprises a second seating area with a second diameter;
• and a bottom surface;
• wherein the outer perimeter of the upper portion is larger than the first diameter of the first seating area of the intermediate portion, and wherein the first diameter is larger than the second diameter of the second seating area of the lower portion;
• and wherein a sample transfer film is applied to the bottom surface of the cap;
• and
• (b) two or more sample chambers, at least one selected from each of:
• (i) a first sample chamber comprising a first opening characterized by a first opening diameter;
• and
• (ii) a second sample chamber comprising a second opening characterized by a second opening diameter that is less than the first opening diameter;
• wherein the first seating area of the intermediate portion of the sample carrier is configured to engage the first opening of the first sample chamber and the second seating area of the lower portion of the sample carrier is configured to engage the second opening of the second sample chamber;
• and
• wherein the cap is either disengaged from both the first sample chamber and the second sample chamber, or the cap is engaged with the first sample chamber, or the cap is engaged with the second sample chamber.